FastQCFastQC Report
Fri 1 Nov 2024
CVFS2_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCVFS2_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206088
Sequences flagged as poor quality0
Sequence length15-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA39911.9365513761111757No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA39411.9122898955785876No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA38621.8739567563370987No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35601.727417413920267No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34931.694907030006599No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34571.6774387640231356No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34231.6609409572609761No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA33731.6366794767283879No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA33671.6337680990644774No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG33411.6211521291875315No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG33391.620181669966228No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32911.5968906486549435No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG32291.5668064127945345No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32211.5629245759093202No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG30331.4717014091067893No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29931.452292224680719No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA29741.4430728620783353No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28941.4042544932261947No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28911.4027988043942392No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28571.3863009976320795No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28351.3756259461977407No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28311.3736850277551338No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28121.3644656651527503No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG27811.3494235472225458No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG26671.294107371608245No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG25451.2349093591087303No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG24761.2014285159737588No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22671.100015527347541No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22491.091281394355809No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22321.083032490974729No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19400.9413454446644152No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19120.9277590155661659No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18980.9209658010170412No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17740.8607973292962231No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17640.8559450331897053No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16870.8185823531695199No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG11070.5371491789914987No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10860.5269593571678118No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA10680.5182252241760802No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10650.5167695353441248No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10630.5157990761228214No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA10520.510461550405652No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA10410.5051240246884826No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA10370.5031831062458756No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10250.4973603509180544No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9450.45854198206591357No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9100.441558945693102No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA9070.4401032568611467No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8930.4333100423120221No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8900.43185435348006673No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8810.4274872869842009No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8700.4221497612670316No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8650.4197236132137728No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8570.4158417763285587No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8480.41147470983269285No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8470.41098948022204107No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8320.40371103606226466No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8310.4032258064516129No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8220.39885873995574705No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA8090.39255075501727416No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7980.3872132293001048No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7760.3765381778657661No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7750.3760529482551143No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7520.3648926672101238No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7390.3585846822716509No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA7160.34742440122666046No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7070.3430573347307946No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG6640.3221924614727689No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG6430.3120026396490819No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG6430.3120026396490819No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5990.2906525367804045No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5980.29016730716975275No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5920.28725592950584217No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5740.27852179651411046No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5420.26299444897325414No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG5280.2562012344241295No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4880.23679204999805908No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4710.22854314661697916No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4650.22563176895306858No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4640.22514653934241685No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4620.2241760801211133No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4590.22272039128915802No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4540.22029424323589922No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4490.21786809518264041No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4470.2168976359613369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4470.2168976359613369No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4400.2135010286867746No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4380.21253056946547105No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4370.21204533985481933No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4250.20622258452699815No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC4230.20525212530569464No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG4070.19748845153526648No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4050.19651799231396294No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG4030.19554753309265946No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4010.19457707387135592No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3960.19215092581809712No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3950.19166569620744536No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3830.18584294087962422No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3780.18341679282636544No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3760.1824463336050619No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3750.18196110399441015No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3750.18196110399441015No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3700.17953495594115135No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG3670.17807926710919605No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3660.1775940374985443No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3640.1766235782772408No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3510.1703155933387679No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3430.16643375645355382No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG3410.16546329723225028No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3370.16352237878964326No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3360.1630371491789915No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3330.16158146034703622No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3310.16061100112573268No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3280.1591553122937774No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG3180.1543030161872598No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3160.1533325569659563No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3150.1528473273553045No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG3140.15236209774465276No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3130.151876868134001No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3090.14993594969139398No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3090.14993594969139398No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3080.1494507200807422No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3040.14750980163813515No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3040.14750980163813515No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG3010.1460541128061799No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3000.14556888319552813No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3000.14556888319552813No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2990.14508365358487638No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2940.14265750553161757No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2940.14265750553161757No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2930.14217227592096582No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2930.14217227592096582No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2890.14023135747835874No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2880.139746127867707No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2880.139746127867707No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG2870.13926089825705523No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2760.13392337253988587No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2750.13343814292923412No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2700.1310119948759753No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2670.12955630604402005No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2640.12810061721206475No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2630.127615387601413No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2620.12713015799076122No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2600.1261596987694577No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2600.1261596987694577No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2580.1251892395481542No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG2500.12130740266294011No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2490.12082217305228835No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2480.12033694344163659No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2460.11936648422033307No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2450.1188812546096813No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2420.117425565777726No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2380.11548464733511898No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2340.11354372889251192No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2300.1116028104499049No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2300.1116028104499049No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2260.10966189200729784No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2190.10626528473273553No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG2140.10383913667947674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2080.10092775901556617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)