FastQCFastQC Report
Fri 1 Nov 2024
CAMS2_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAMS2_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165995
Sequences flagged as poor quality0
Sequence length69-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT80354.840507244194102No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT77994.698334287177325No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT73134.405554384168198No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT71634.3151902165727885No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT71264.29290038856592No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT70364.238681888008674No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT68914.151329859333113No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT60103.6205909816560737No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24131.4536582427181541No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC24011.4464291093105213No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC23391.4090785867044189No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23191.397030031025031No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22771.3717280640983163No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22471.3536552305792342No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22451.3524503750112955No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22261.341004247115877No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22091.3307629747883973No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21401.289195457694509No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21101.271122624175427No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21011.2657007741197024No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC21001.265098346335733No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT20861.2566643573601615No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC20421.2301575348655078No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT20371.2271453959456609No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT19541.1771438898762012No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19121.1518419229494865No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT19111.151239495165517No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT18561.1181059670472002No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC18171.0946112834723936No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17311.0428024940510257No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17021.0253320883159132No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT16400.9879815657098105No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC13950.8403867586373083No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC13640.8217114973342571No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC13370.8054459471670834No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC13360.804843519383114No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12940.7795415524563993No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12840.7735172746167053No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12620.7602638633693786No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10760.6482122955510707No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC9160.5518238501159674No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC8980.5409801500045182No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC8410.5066417663182626No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC7900.4759179493358233No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC7790.46929124371215997No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC7430.44760384348926174No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC7410.4463989879213229No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC6350.38254164282056685No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5150.31025030874423926No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC5130.3090454531763005No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5060.3048284586885147No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4870.29338233079309617No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4740.28555076960149406No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4720.28434591403355525No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4590.2765143528419531No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4550.2741046417060755No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4550.2741046417060755No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4530.2728997861381367No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4340.26145365824271816No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4270.25723666375493237No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4190.25241724148317723No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4180.25181481369920783No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4140.2494051025633302No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4120.24820024699539145No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3810.22952498569234012No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3700.2228982800686768No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3620.21807885779692157No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3600.21687400222898282No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3600.21687400222898282No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3520.21205457995722762No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3380.20362059098165608No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3290.19819874092593148No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3280.1975963131419621No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3230.19458417422211513No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3150.18976475195035997No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3090.18615018524654356No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3040.18313804632669659No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3020.1819331907587578No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2900.17470405735112501No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2850.17169191843127804No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2780.16747492394349228No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2660.16024579053585952No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2530.15241422934425736No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2500.15060694599234917No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2450.1475948070725022No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2340.1409681014488388No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2320.13976324588090003No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2150.12952197355342027No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2060.12410012349769572No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2040.12289526792975691No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1950.11747341787403236No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1930.11626856230609356No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1930.11626856230609356No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC1900.11446127895418537No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1780.1072321455465526No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1730.10422000662670561No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1670.10060543992288926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)