FastQCFastQC Report
Fri 1 Nov 2024
CAFS2_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAFS2_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150374
Sequences flagged as poor quality0
Sequence length225-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34622.302259699150119No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34412.288294518999295No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA33752.244403952810991No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA29751.9784005213667257No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA29411.955790229693963No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG28521.8966044661976138No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA28141.8713341402104087No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA27521.8301036083365474No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG27321.8168034367643342No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG27011.7961881708274037No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA26901.7888730764626863No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA24431.6246159575458523No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24191.6086557516591964No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG24091.6020056658730897No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23591.5687552369425566No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG23371.554125048213122No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG23241.5454799366911833No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG22671.5075744477103754No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG22381.4882891989306661No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20901.3898679292962879No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20531.3652626118876934No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20161.3406572944790986No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20011.3306821657999388No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19531.298761754026627No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19471.294771702554963No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18971.2615212736244297No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17801.1837152699269822No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17651.173740141247822No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17441.1597749610969983No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17171.1418197294745103No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15000.997512867915996No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14820.9855427135010041No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13910.9250269328474336No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13700.9110617526966097No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13510.8984265897030072No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12680.843230877678322No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA10090.6709936558181601No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9570.6364132097304055No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9480.6304281325229095No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9390.6244430553154136No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8890.5911926263848803No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8740.5812174977057204No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8680.5772274462340564No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8620.5732373947623924No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8590.5712423690265604No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8230.5473020601965766No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8230.5473020601965766No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8090.5379919400960272No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8050.5353319057815845No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7960.5293468285740885No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7910.5260217856810353No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7530.5007514596938301No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7290.4847912538071741No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC7120.4734861079707929No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7080.47082607365635015No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC7080.47082607365635015No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7070.47016106507773947No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6800.45220583345525156No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6720.44688576482636627No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6640.44156569619748093No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6250.41563036163166506No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6230.41430034447444375No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6150.4089802758455584No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6090.4049902243738944No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5940.3950150956947345No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5830.38770000133001714No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5810.38636998417279583No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5740.38171492412252117No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5720.38038490696529986No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5710.3797198983866892No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5580.37107478686475054No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5500.36575471823586525No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5470.3637596925000332No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5410.3597696410283692No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5370.3571096067139266No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5300.352454546663652No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5150.342479417984492No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5100.3391543750914387No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5060.336494340776996No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4760.3165440834186761No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4720.31388404910423345No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4660.3098939976325695No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4640.3085639804753481No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4630.3078989718967375No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4530.30124888611063083No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4440.2952638089031348No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4430.2945988003245242No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4390.29193876601008156No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4380.2912737574314708No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4300.2859536888025856No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4290.28528868022397486No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4280.28462367164536423No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4250.28262864590953224No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4240.28196363733092156No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4050.26932847433731893No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4010.26666844002287626No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4000.26600343144426564No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3940.26201337997260166No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3930.261348371393991No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3900.259353345658159No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3890.2586883370795483No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3860.2566933113437163No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3850.2560283027651057No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3830.2546982856078843No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3610.2400680968784497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3570.23740806256400707No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3560.23674305398539644No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3540.23541303682817508No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3520.23408301967095374No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3470.23075797677790041No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3460.23009296819928976No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3320.22078284809874046No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3270.21745780520568714No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3260.21679279662707648No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3220.21413276231263384No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3140.20881269368374852No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3030.20149759931903122No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3000.19950257358319923No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2980.1981725564259779No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2680.178222299067658No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2670.1775572904890473No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2550.16957718754571935No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2470.16425711891683403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2470.16425711891683403No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2460.16359211033822335No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2330.15494699881628474No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2310.1536169816590634No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2290.15228696450184206No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2270.15095694734462076No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2140.14231183582268211No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2010.13366672430074347No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG1980.13167169856491148No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG1930.12834665567185816No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG1910.12701663851463685No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG1680.11172144120659157No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1520.10108130394882094No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1510.10041629537021028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)