FastQCFastQC Report
Fri 1 Nov 2024
CAFS1_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAFS1_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162608
Sequences flagged as poor quality0
Sequence length38-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA42922.639476532519925No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA42482.612417593230345No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA41502.552149955721736No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36242.2286726360326674No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36232.228057660139722No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34842.142576011020368No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA34522.1228967824461282No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA32602.004821411000689No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA30541.8781363770540196No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG29931.8406228475843747No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG28791.7705155957886451No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG27901.7157827413165405No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG24531.5085358653940766No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG24401.5005411787857916No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23851.4667175046738168No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23201.426744071632392No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG22871.406449867165207No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22291.3707812653743974No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22161.3627865787661124No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20921.2865295680409328No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20571.265005411787858No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19981.228721834104103No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19901.2238020269605432No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19851.2207271474958181No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19621.2065827019580833No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19191.1801387385614486No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18911.1629194135589886No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17381.068828101938404No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17201.057758535865394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17131.0534537046147792No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17101.0516087769359441No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16741.0294696447899243No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16521.0159401751451342No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15970.9821165010331595No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15680.9642822001377547No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15260.9384532126340648No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9820.6039063268719866No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9640.5928367607989766No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9510.5848420741906917No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9400.5780773393682967No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9230.5676227491882319No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9120.5608580143658368No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8570.5270343402538621No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8340.5128898947161271No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8230.5061251598937322No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8180.5030502804290072No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8070.4962855456066122No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7910.4864459313194923No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7890.4852159795336023No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7800.47968119649709734No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7680.47230148578175735No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7430.4569270884581324No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7380.45385220899340745No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7240.4452425464921775No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7210.44339761881334255No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG7130.43847781166978256No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7110.43724785988389253No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6950.42740824559677265No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6620.40711404112958777No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6600.40588408934369774No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6460.3972744268424678No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6270.38558988487651286No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA6180.3800551018400079No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG6150.3782101741611729No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG6120.3763652464823379No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5980.36775558398110797No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5970.36714060808816296No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5940.36529568040932797No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5770.354841090229263No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5710.35115123487159305No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5650.3474613795139231No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5630.34623142772803306No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5580.3431565482633081No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5580.3431565482633081No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5510.3388517170126931No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5430.3339319098691331No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5430.3339319098691331No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5400.33208698219029814No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5360.32962707861851814No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5340.3283971268326281No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5230.3216323920102332No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5220.3210174161172882No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG5210.32040244022434317No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG5050.31056282593722323No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5050.31056282593722323No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5030.30933287415133326No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5020.30871789825838825No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4950.30441306700777326No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4920.3025681393289383No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4880.3001082357571583No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4850.2982633080783233No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA4740.2914985732559284No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4740.2914985732559284No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4670.2871937420053134No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4660.2865787661123684No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4640.2853488143264784No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4450.2736642723605235No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4420.2718193446816885No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4420.2718193446816885No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG4400.2705893928957985No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4360.2681294893240185No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4340.2668995375381285No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4090.2515251402145036No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4080.2509101643215586No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4080.2509101643215586No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4070.2502951884286136No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG4060.24968021253566863No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3970.24414542949916362No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3970.24414542949916362No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3910.24045557414149366No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3900.23984059824854867No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3900.23984059824854867No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3790.2330758634261537No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3750.2306159598543737No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3730.22938600806848372No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3720.2287710321755387No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3680.2263111286037587No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3540.2177014661025288No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3440.21155170717307883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3410.20970677949424385No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG3390.20847682770835382No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3220.19802223752828887No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG3200.1967922857423989No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3120.19187247859883894No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG3070.18879759913411395No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2830.17403817770343402No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2830.17403817770343402No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2800.17219325002459904No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2780.17096329823870904No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2770.17034832234576405No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2750.16911837055987405No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2690.16542851520220406No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2650.1629686116304241No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGTGCCTCAG2570.15804880448686412No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG2440.15005411787857914No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG2150.13221981698317425No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1880.11561546787365935No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1810.11131063662304437No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1780.10946570894420939No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG1700.10454590180064942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)